The new Gaussian parameters from Helios
Hi,
Recently changed from a CyTOF1 to a the Helios system. I can't find much information on the 4 new parameters added to FCS files by Helios - width, residual, centre and offset. Could anyone point me in the direction of where these are explained or how we can use them to gate out ion fusion events? Is it as simple as just gating the large population as in the attached 2 plots? Do people now use these routinely as part of their gating strategy?
Many thanks,
David.
Recently changed from a CyTOF1 to a the Helios system. I can't find much information on the 4 new parameters added to FCS files by Helios - width, residual, centre and offset. Could anyone point me in the direction of where these are explained or how we can use them to gate out ion fusion events? Is it as simple as just gating the large population as in the attached 2 plots? Do people now use these routinely as part of their gating strategy?
Many thanks,
David.