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Matlab Normalizer

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skowli

Participant

Posts: 2

Joined: Wed Apr 05, 2017 12:01 am

Post Thu Apr 06, 2017 5:01 pm

Matlab Normalizer

Hi,

I normalized my fcs files using a same referral normalized fcs file for two independent batches. For one batch "before" and "after" graphs were generated but for the other batch there was no graphs generated in the "after" normalization plot. Not sure what is the issue? Any inputs would be greatly appreciated. My email is skowli@stanford.edu

Thank you,
Sangeeta
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mleipold

Guru

Posts: 2162

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Thu Apr 06, 2017 5:12 pm

Re: Matlab Normalizer

Hi Sangeeta,

Could you give us more detail?
1) Examples of the "before and after" plots for each batch (including the no-graph one)?
2) Examples of what the data looked like afterward (FlowJo or Cytobank), for both good and bad?
3) Any other data you could give (example FCS files: before norm, after norm, your "referral" file if used for both), etc


Thanks,
Mike
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skowli

Participant

Posts: 2

Joined: Wed Apr 05, 2017 12:01 am

Post Thu Apr 06, 2017 5:48 pm

Re: Matlab Normalizer

Hi Mike,

I have attached a pdf which includes

1. the normalization "before" and "after" graphs for both batches
2. the FlowJo gates (before and after normalization) using one file from each batch.

Thanks,
Sangeeta
normalization plots_sk.pdf
(390.65 KiB) Downloaded 196 times

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