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Negatives split in 4

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marodon

Participant

Posts: 3

Joined: Mon Sep 26, 2016 7:46 am

Post Thu Dec 08, 2016 10:37 am

Negatives split in 4

Hello all, a naïve question (maybe not): why is there 4 negatives subsets in a cytof fcs tube? How to deal with cells positive for one marker that split in two on the other axis? Transformation? But doesn't that create subsets that do not exist or eliminate true negatives? I don't like to play around with signals after acquisition. Thanks for you help.
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mleipold

Guru

Posts: 2162

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Thu Dec 08, 2016 4:00 pm

Re: Negatives split in 4

Hi Marodon,

I'm not sure I understand what you're talking about. Could you show us some example data, including information on the panel you used, model of instrument, and type of sample (PBMCs, whole-blood, etc)?


-Mike

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