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CytoNORM question

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marthabrainard1

Contributor

Posts: 20

Joined: Wed Aug 25, 2021 2:10 pm

Post Wed Jun 29, 2022 3:46 pm

CytoNORM question

Hi everyone,

I am very new to CytoNORM and was wondering if it possible to use on CyTOF data if you have sample spike-in controls rather than one batch control and how this would work? Would you select one spike-in from every experiment?

Thank you,
Martha
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tomash

Contributor

Posts: 24

Joined: Sun Oct 19, 2014 10:15 pm

Post Tue Aug 02, 2022 10:30 am

Re: CytoNORM question

Hi Martha,

Excellent question -- do you have more info on what is in the spike-in control in your setting?

Tom
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marthabrainard1

Contributor

Posts: 20

Joined: Wed Aug 25, 2021 2:10 pm

Post Tue Aug 02, 2022 5:16 pm

Re: CytoNORM question

Hi Tom, Our reference cells include stimulated PBMCs and drug product in 1:2 ratio. Our samples are drug product, patient PBMCs, or healthy donor unstimulated PBMCs.

Martha

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