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CytoNORM question

PostPosted: Wed Jun 29, 2022 3:46 pm
by marthabrainard1
Hi everyone,

I am very new to CytoNORM and was wondering if it possible to use on CyTOF data if you have sample spike-in controls rather than one batch control and how this would work? Would you select one spike-in from every experiment?

Thank you,
Martha

Re: CytoNORM question

PostPosted: Tue Aug 02, 2022 10:30 am
by tomash
Hi Martha,

Excellent question -- do you have more info on what is in the spike-in control in your setting?

Tom

Re: CytoNORM question

PostPosted: Tue Aug 02, 2022 5:16 pm
by marthabrainard1
Hi Tom, Our reference cells include stimulated PBMCs and drug product in 1:2 ratio. Our samples are drug product, patient PBMCs, or healthy donor unstimulated PBMCs.

Martha