Quantifying Metal Mass on EQ Beads
Posted: Wed Jan 13, 2021 11:42 am
I am trying to obtain the masses for Ce140, Eu153, Ho165, and Lu175 on the EQ beads. I ran dissolved metal standards for these elements at known concentrations to establish a calibration curve in solution mode. I then ran the EQ beads in event mode. I hypothesized that if I have known ion counts from the standards I can obtain the mass of metals on the beads.
With the data I have now, I can calculate the TE for the dissolved standards. From there I am kind of lost. I am following the equations/rationale in this paper where they quantified silver nanoparticles per cell. https://pubs.acs.org/doi/10.1021/acs.analchem.7b01006.
Another study I am trying to follow is here: https://pubs.acs.org/doi/10.1021/ja9052009 where they calculated the number of metal ions on microspheres with CyTOF.
Any thoughts or insight is greatly appreciated! Thank you
With the data I have now, I can calculate the TE for the dissolved standards. From there I am kind of lost. I am following the equations/rationale in this paper where they quantified silver nanoparticles per cell. https://pubs.acs.org/doi/10.1021/acs.analchem.7b01006.
Another study I am trying to follow is here: https://pubs.acs.org/doi/10.1021/ja9052009 where they calculated the number of metal ions on microspheres with CyTOF.
Any thoughts or insight is greatly appreciated! Thank you