Concatenating barcoded samples from different runs
Dear all,
I have a set of files from multiple acquisitions of the same pool of barcoded samples (14 clinical samples and 1 reference). We split the pool of samples to multiple vials, froze them, and then thaw on the day of acquisition. Here's how the CD3 expression looked like across the acquisitions. I do not think there's a prominent shift of marker expression distributions, but I can see that the lines (which indicate quantiles) are not aligned. What would be the best way to proceed?
https://ibb.co/X2NbjWc
Would it be wise to concatenate all files and then debarcode them?
I appreciate all of your insights.
Best regards,
Mikhael
I have a set of files from multiple acquisitions of the same pool of barcoded samples (14 clinical samples and 1 reference). We split the pool of samples to multiple vials, froze them, and then thaw on the day of acquisition. Here's how the CD3 expression looked like across the acquisitions. I do not think there's a prominent shift of marker expression distributions, but I can see that the lines (which indicate quantiles) are not aligned. What would be the best way to proceed?
https://ibb.co/X2NbjWc
Would it be wise to concatenate all files and then debarcode them?
I appreciate all of your insights.
Best regards,
Mikhael