Auto-gating Gaussian and/or cells?
Is there any appetite for a script that auto-gates CyTOF data using the Gaussian parameters and/or beads?
Because I like doing this sort of thing, I wrote one anyway...
UPDATE - now exists as an R package - https://github.com/JimboMahoney/cytofclean
It outputs plots to show the gates used and the %events remaining and then writes new FCS files containing only the events of interest.
Each row is a gate (Event -> Centre -> Offset -> Residual -> Cells) and each column is a file.
Of course, as with any automated task, there is a risk of it going wrong, but at least with this script, it shows you how it's gated the events and also outputs NEW files, rather than overwriting the originals.
And of course, this is WAY faster than doing it manually...