Many ways to skin an isotope. What's yours? Data sharing in the form of FCS files and images is encouraged.
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Please be as geeky as possible. Reference, reference, reference. Also, please note that this is a mixed bag of math-gurus and mathematically challenged, so choose your words wisely
I played around with it quite a bit. I presume you are referring to the R package? My biggest complaint is that it is (or at least was) not designed for use with CyTOF data, only flow. I found that by changing the code to test coefficient values around 5, it worked much better.
Thanks for sharing. Yes, it is R package and I am planning to use it for Cytof. I tried to play with it but keep getting "Error in optimize(afun, b, maximum = TRUE) : 'xmin' not less than 'xmax'.