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FlowVS for transformation

PostPosted: Thu May 02, 2019 2:56 pm
by YWzhang
Does anyone here tried FlowVS for cytof transformation? Dose it make difference? Any experience to share?

Re: FlowVS for transformation

PostPosted: Tue May 07, 2019 5:36 pm
by bc2zbUVA
I played around with it quite a bit. I presume you are referring to the R package? My biggest complaint is that it is (or at least was) not designed for use with CyTOF data, only flow. I found that by changing the code to test coefficient values around 5, it worked much better.

Re: FlowVS for transformation

PostPosted: Mon May 13, 2019 4:06 pm
by YWzhang
Thanks for sharing. Yes, it is R package and I am planning to use it for Cytof. I tried to play with it but keep getting "Error in optimize(afun, b, maximum = TRUE) : 'xmin' not less than 'xmax'.