Post Mon Sep 24, 2018 10:14 pm

autogating

Hi guys,

I was trained in the immunology field and was taught to set up the standard gating and apply the same gating to every sample for comparison. However, our collaborator is trying to help us develop autogating system, but they used personalized gating for the individual sample (I understand why to do personalized gating). Is there anybody who could provide a suggestion or discussion about which way of gating is better, especially for cytof data?

Because gating is a super important first step, I want to learn more from all of you.

Thank you!!

Xiao