Catalyst Pre-Processed file in Cytobank
I've been playing around with the CATALYST package in R and was wondering if anyone else has had an issue with uploading the concatenated/normalized/debarcoded fcs files onto Cytobank? It seems as there are two of every keyword in my FCS Header Text and Cytobank picks the earliest one. This doesn't seem like an issue, except it chooses total events from the master file (say 1 million events) but the individual debarcoded file is only 200k events. This makes the files unusable in Cytobank as they see a discrepancy in event numbers and the $TOT keyword. Any help with this would be much appreciated!
-Chad