TIMESTEP for Cytof data
Does anyone have a proper strategy for this? What is a proper value to add? Is this fixed for Cytof data?
I would appreciate some expert suggestions.
Many thanks in advance,
Costa
BjornZ wrote:Hi Costa -- I think the correct value is 0.001 (that is, one TIME unit is 1 millisecond). That gives reasonable values for data I just looked at from a Helios and CyTOF2.
jimbomahoney wrote:I can't help with the flowAI, but I can also confirm that 1ms is correct.
I've written a script that plots FCS data vs. time and my script implements 10 ms units for flow data and 1 ms units for CyTOF data.
The results are correct for BD instruments (Canto, Aria) and CyTOF 2 and 3 (Helios) - i.e. the event rate and total acquisition duration are correct in each case.
jimbomahoney wrote:Sure - please excuse the probably messy code! I'm new to R and cytometry!
https://github.com/JimboMahoney/CytobankGraphs
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