Post Tue Dec 14, 2021 10:15 pm

Tuning for mass imaging

Hi everyone,

Hope you are all OK despite COVID - here in Australia we are just opening up after various lockdowns, so cases are up 10-fold and rising, and we have a huge superspreader event where >200 people were infected. Crossing our fingers that not too many of our parents and grandparents die over Xmas and the New Year.

I am not familiar with the IMC tuning procedure from a practical point of view - but a question about tuning has just arisen in data from one of our collaborators.

We recently did a run where the machine stopped half way through an ROI (I don’t know why). When the remaining half of the ROI was run the next morning (Friday), the counts in all the channels averaged approx 2x higher, but there were very high counts in 155 (9x higher) and 165 (4.5x higher), - so it looked as though we had a lot of oxidation happening. We could also see that on Friday the 139 signal (which was in the cell nucleus) was also in 155 (which was an antibody that does not stain the nucleus), whereas it had not been there on Thursday - so we could actually see that the staining pattern from 139 (nuclear) was seen in 155 on Friday but not on the previous day.

The tuning results provided by the facility staff were

Lu175 MD Xe131 MD Trans CT1 Trans CT2
2021-10-28 Thur 1833 801 0.0125 0.0023
2021-10-29 Friday 1718 911 0.0124 0.0023

I don’t do imaging much, but keeping track of 16+ signals is part of the tune on suspension mode - is this also controlled in imaging mode using the tuning slide that has only 89Y, 140Ce and 175Lu?

?Are all the tuning details kept on the hard drive - can they be looked at to see if there was a problem with 16+ signals on the Friday run?

Thanks for your help

Ramaciotti Facility, Sydney, Australia