Post Sat Sep 28, 2019 3:17 am

Research Assistant at the University of Colorado

Please apply through this website:
https://cu.taleo.net/careersection/2/jo ... Eg.twitter

Please email elena.hsieh@cuanschutz.edu if you have additional questions.

Nature of Work:
A professional research assistant full-time position is available in the Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus. The research focus of the laboratory is to study autoimmunity, inflammation, and primary immunodeficiency. This research position will involve laboratory-based studies with human and mouse samples performed at biosafety level 2.

Duties and Responsibilities: The successful candidate will participate in research projects and is expected to help maintain the operation of the laboratory. Work will largely be experimental, and may require up to 10% clerical and managerial activities. Work duties include, but are not limited to:

Multicolor flow cytometry: design, optimization and analysis in BD instruments. Knowledge of DIVA and Flowjo platforms. Knowledge of spectral cytometry is desired but not required.
Knowledge of mouse and human immunology.
CyTOF knowledge is desired but not required
Analyze experimental results and prepare data for publication in peer-reviewed journals and for presentation at scientific meetings. Includes literature search, taking the lead on preparation of manuscript, final submission and any subsequent revisions for publication.
Review, develop and implement new or modified laboratory techniques required for research projects. Includes trouble-shooting, problem-solve work, and keeping a detailed laboratory notebook, accurately collecting records of experiments.
Participate in lab meetings presenting data/ journal club
Project and database management. Includes data-mining human/mouse sample inventories, tracking and documenting chemicals use,
Maintain all applicable laboratory safety certifications, includes: follow all UC Denver guidelines regarding the management of chemical, radiochemical and infectious waste and ensuring that all lab personnel meet and maintain biosafety and occupational health requirements
Manage high school/undergraduate interns, rotating graduate students and other lab personnel. Assign duties as needed
Prepare and update protocols for animal ethics committees

Minimum Qualifications: Applicants should hold a BS degree in life sciences or related field, or have equivalent experience.

Preferred Qualifications: Experience and background in molecular biology, cellular immunology (flow cytometry), and/or working with mice are highly desired. In addition, the applicant should be able demonstrate their ability to work independently and as a member of a team.

* Applicants with a master’s degree and/or 6 years of experience with the duties and responsibilities and techniques listed above may be hired at the Senior Professional Research Assistant level

Techniques

Tissue culture
Flow cytometry: design, optimization, staining and analysis (DIVA, FlowJo, Cytobank). single-cell sorting is desired but not required.
Molecular biology: PCR, qPCR, primer design. Designing complex mouse genotyping. DNA and RNA extraction and purification. Cloning. CRISPR is desirable but not required.
Mouse work: design of infection models/experiments. Survival curves. Able to inject IV and IP. Mouse colony managing and handling. Designing genotyping PCRs for simple and complex strains. Dissections and organ identification and processing for flow cytometry/CyTOF/qPCR.
Computer software or technical skills: Knowledge of other statistical software including Graphpad Prism and/or SAS or SPSS. Understanding of R and/or Matlab programming greatly beneficial but not required.