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Metal-Conjugated NeutrAvidin for MHC Multimer Assays

Would you perform tetramer assays in your lab / core?

I already perform tetramer assay.
1
20%
I would if there was a kit I could purchase.
4
80%
I would if there was a do-it-yourself protocol.
0
No votes
I am not interested in tetramer assays.
0
No votes
 
Total votes : 5
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Ofir

Master

Posts: 75

Joined: Thu Nov 07, 2013 12:46 pm

Location: US, CA

Post Sat Feb 27, 2016 6:27 pm

Metal-Conjugated NeutrAvidin for MHC Multimer Assays

This Fluidigm poster showcases metal-tagged tetramer assays for detection of CMV-specific T cells in PBMCs.
https://www.mblintl.com/assets/AAI-Post ... -final.pdf
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mleipold

Guru

Posts: 2086

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Mon Feb 29, 2016 4:09 pm

Re: Metal-Conjugated NeutrAvidin for MHC Multimer Assays

Hi Ofir,

Are there plans for Fluidigm to sell the "proprietary amine-reactive metal-chelating polymer" mentioned in this poster?

Aside from the issue that some antibodies are happier with amine-chemistry, there are a lot of other proteins/probes out there which don't have thiol/Cys (eg, some of the lectins I used back at UofT).


Mike

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