Post Thu Apr 20, 2017 7:02 pm

Staining controls

I'm relatively new to mass cytometry and was wondering what you guys typically use for a staining control when running a whole blood panel. Since neutrophils don't freeze well, we need to obtain fresh blood each time we stain. Additionally, we are only truly interested in running patient/disease blood (which we may only get 1 or 2 samples at a time) and do not need data from healthy controls. For now, we plan on drawing blood from a healthy donor each time we stain patient blood. But is there a better and easier option? Thanks in advance for everyone's help!