Fri Jan 19, 2024 1:24 am by ycheng
Hi,
The sample introduction system of Helios is quite simple and straightforward, so it's easy to handle when there is a clot. I just carefully and thoroughly backflash (with MilliQ or wash buffer) the loop connected with nebulizer capillary loop and autosampler before and after every run. I also tested out different nebulizer and capillary loop (they are different between different batches), and use the best ones for important experiment. When I see the event rate drop sharply, I stop the run and clean all the loop and resume the acquisition. Yes...I usually staring at the monitor (instead of walking away or using teamviewer) to make sure the acquisition rate is ok, at least for important experiment.
I acquire blood samples at 500K-650K cell/ml, and lower rate for tissue samples, around 350K-500K cell/ml.
Hope this helps.
Yang