inner diameter for sample line sleeves/capillary
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Hello- does anyone have the sizes for the sleeves and capillary that are used to assemble the sample lines for CyTOF 2?
Re: inner diameter for sample line sleeves/capillary
HI Trevor,
I don't have any info on the blue sleeves; I just buy packs of them from Fluidigm as needed. While you do lose a small bit every time you recut when rebuilding the capillary, it lasts for a long time.
Regarding the capillary itself: when we make our own, we've been using a 100ft spool of Cole-Parmer PTFE Special Tubing Natural item #06417-74 ( https://www.fishersci.com/shop/products ... k/50634338 )
If my math is right:
0.006" ID = 152um
0.016" OD = 406um
Please note for Helios users: this ~150um ID will give you a PSI pressure of 4-5psi. The Fluidigm capillaries are ~100um ID, which give the PSI pressure of ~10psi.
For years, we had been using a 0.004" ID spool (which would be ~100um): viewtopic.php?f=4&t=613&p=1892&hilit=parmer#p1892
However, something changed with that product: the OD seemed to get narrower after a certain point, which made things start slipping out of the blue sleeves ( viewtopic.php?f=4&t=1423&p=4065&hilit=parmer#p4065 ).
That's when we changed to the 0.006" ID one; supposedly the same OD, but that one doesn't slip out of the blue sleeves.
Mike
I don't have any info on the blue sleeves; I just buy packs of them from Fluidigm as needed. While you do lose a small bit every time you recut when rebuilding the capillary, it lasts for a long time.
Regarding the capillary itself: when we make our own, we've been using a 100ft spool of Cole-Parmer PTFE Special Tubing Natural item #06417-74 ( https://www.fishersci.com/shop/products ... k/50634338 )
If my math is right:
0.006" ID = 152um
0.016" OD = 406um
Please note for Helios users: this ~150um ID will give you a PSI pressure of 4-5psi. The Fluidigm capillaries are ~100um ID, which give the PSI pressure of ~10psi.
For years, we had been using a 0.004" ID spool (which would be ~100um): viewtopic.php?f=4&t=613&p=1892&hilit=parmer#p1892
However, something changed with that product: the OD seemed to get narrower after a certain point, which made things start slipping out of the blue sleeves ( viewtopic.php?f=4&t=1423&p=4065&hilit=parmer#p4065 ).
That's when we changed to the 0.006" ID one; supposedly the same OD, but that one doesn't slip out of the blue sleeves.
Mike
Re: inner diameter for sample line sleeves/capillary
If you wanted to measure what you have, take a thin slice and photograph the section on a hemocytometer. The grid lines are standard distances that you can use to related the mm/pixel to the diameter in pixels on the image.
For example: https://photos.app.goo.gl/1CnKmhkVaiNRuLcC9
Greg Chang
For example: https://photos.app.goo.gl/1CnKmhkVaiNRuLcC9
Greg Chang
Re: inner diameter for sample line sleeves/capillary
Thank you all!
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