FAQ  •  Register  •  Login

inner diameter for sample line sleeves/capillary

<<

trevorbruce

Participant

Posts: 5

Joined: Wed Oct 06, 2021 9:56 pm

Post Tue Nov 02, 2021 5:27 pm

inner diameter for sample line sleeves/capillary

Hello- does anyone have the sizes for the sleeves and capillary that are used to assemble the sample lines for CyTOF 2?
<<

mleipold

Guru

Posts: 5796

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Tue Nov 02, 2021 5:42 pm

Re: inner diameter for sample line sleeves/capillary

HI Trevor,

I don't have any info on the blue sleeves; I just buy packs of them from Fluidigm as needed. While you do lose a small bit every time you recut when rebuilding the capillary, it lasts for a long time.

Regarding the capillary itself: when we make our own, we've been using a 100ft spool of Cole-Parmer PTFE Special Tubing Natural item #06417-74 ( https://www.fishersci.com/shop/products ... k/50634338 )

If my math is right:
0.006" ID = 152um
0.016" OD = 406um

Please note for Helios users: this ~150um ID will give you a PSI pressure of 4-5psi. The Fluidigm capillaries are ~100um ID, which give the PSI pressure of ~10psi.

For years, we had been using a 0.004" ID spool (which would be ~100um): viewtopic.php?f=4&t=613&p=1892&hilit=parmer#p1892
However, something changed with that product: the OD seemed to get narrower after a certain point, which made things start slipping out of the blue sleeves ( viewtopic.php?f=4&t=1423&p=4065&hilit=parmer#p4065 ).

That's when we changed to the 0.006" ID one; supposedly the same OD, but that one doesn't slip out of the blue sleeves.


Mike
<<

Chowduck

Contributor

Posts: 29

Joined: Wed Nov 19, 2014 4:39 pm

Post Tue Nov 02, 2021 5:45 pm

Re: inner diameter for sample line sleeves/capillary

If you wanted to measure what you have, take a thin slice and photograph the section on a hemocytometer. The grid lines are standard distances that you can use to related the mm/pixel to the diameter in pixels on the image.
For example: https://photos.app.goo.gl/1CnKmhkVaiNRuLcC9

Greg Chang
<<

trevorbruce

Participant

Posts: 5

Joined: Wed Oct 06, 2021 9:56 pm

Post Tue Nov 02, 2021 11:25 pm

Re: inner diameter for sample line sleeves/capillary

Thank you all!

Return to CyTOF general discussion

Who is online

Users browsing this forum: No registered users and 10 guests