Live cell barcoding and intracellular staining
1. Do you skip palladium barcoding fix/perm steps or do these incubations somehow prep the cells for the harsher eBio fix/perm? We wouldn't be adding palladium barcodes, but are wondering if anyone has QC'd getting rid of these steps and how that affects sample quality and intracellular staining
2. Have you been able to shorten the eBio fix/perm incubation
3. Have you used any other nuclear staining/transcription factor kits other than eBio
4. Have you skipped the palladium barcoding fix/perm steps and used a less harsh fix/perm (BD cytofix/cytoperm)