Doublets vs. G2 cells
Posted: Mon May 11, 2020 3:33 pm
Hi all,
I have a rather simple question: How do you actually gate single cells based on the Ir signal? Usually, there is a big population representing the singlets and a second, smaller population supposedly representing the doublets. But is this "doublet" population not consisting of both doublets of G1 cells as well as single G2 cells? If one prepares a well dispersed cell suspension with a relatively low concentration, would one harm the dataset more by removing G2 cells than actually correcting it?
Just to hear your thoughts and how you handle this. I was taught to gate on that "G1 population" but I'm not sure that's a good procedure.
Best,
Gero
I have a rather simple question: How do you actually gate single cells based on the Ir signal? Usually, there is a big population representing the singlets and a second, smaller population supposedly representing the doublets. But is this "doublet" population not consisting of both doublets of G1 cells as well as single G2 cells? If one prepares a well dispersed cell suspension with a relatively low concentration, would one harm the dataset more by removing G2 cells than actually correcting it?
Just to hear your thoughts and how you handle this. I was taught to gate on that "G1 population" but I'm not sure that's a good procedure.
Best,
Gero