Thu Aug 22, 2019 4:13 pm by dtdavani
Hi All,
I hope this is not to late! Back in the day when I was at Toronto SickKids core we had the same problem ( I miss CYTOF challenges;).
This happens when the windows JAVA is updated CYTOF software cannot find it's default JAVA. here is the solution Fluidigm kindly provided us. I hope it works. Sorry pics did not copy so I have attached the PDF.
loos of Guassian distribution parameters:
This is caused by Java problem (Windows’ Java update)
Do not update Java on Helios computers. If happens:
• To add Gaussian discrimination parameters, you will need to have the IMD files for the acquisitions and you will need the correct Java path on the computer you are processing this. Please disable automatic Java updates if it is enabled, as the Java path in the instrument software is not automatically updated.
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• Follow these steps:
• Click on the Process Tab.
• Click on IMD reprocessing. This window will open:
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• In Source file, load the IMD file that you wish to convert.
• Ensure that the Gaussian Discrimination checkbox is checked:
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• Also make sure that the Lower Convolution Threshold value matches with the one in the template with which the file is acquired. You can find that information in Experiment Manager once you load up the template (enable Advanced settings):
•
•
Once ready, click Start in the IMD processing window. The FCS will start being generated.