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Characterizing our own instrument

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Verhoeff

Participant

Posts: 19

Joined: Wed Mar 02, 2016 10:25 am

Post Mon Apr 29, 2019 3:01 pm

Characterizing our own instrument

Hi all,

In our lab we were wondering if we can accurately quantify what the differences in mass sensitivity are between metals. We've seen the graph provided by Fluidigm on which metals yield the highest signals, but we reckon there will be slight differences between instruments.

Now we found a Rare earth element mix for ICP from Merck (product # 67349) and that might prove useful. Has anyone tried running an ICP solution to characterize their own machine?


Jan
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mleipold

Guru

Posts: 5796

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Mon Apr 29, 2019 3:41 pm

Re: Characterizing our own instrument

Hi Jan,

I personally would recommend the Multielement solution used in Tricot et al (http://dx.doi.org/10.1002/cyto.a.22648).

I have previously posted our characterization of several of our machines: viewtopic.php?f=7&t=942&p=2781&hilit=tricot+et+al#p2781 (Tue Feb 06, 2018 12:55 pm post, with the attached PDF).


Mike
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dtelad11

Master

Posts: 129

Joined: Mon Oct 31, 2016 6:26 pm

Post Mon Apr 29, 2019 3:42 pm

Re: Characterizing our own instrument

I've been talking to Adeeb Rahman of the Sinai IMC about a similar idea. BioLegend offers a product called Veri-Cells which are a lyophilized cell standard:

https://www.biolegend.com/en-us/product ... pbmc-12260

And Fluidigm recently released their lyophilized, standardized immune monitoring panel:

https://www.fluidigm.com/binaries/conte ... igm%3Afile

Finally, my company, Astrolabe, offers a standardized platform for labeling and analyzing mass cytometry data:

https://www.astrolabediagnostics.com/

Adeeb and I discussed combining all of the above to create an immunophenotyping fingerprint for a mass cytometry instrument. The general idea is that you'll run 20-30k Veri-Cells with the MaxPar panel every morning and use that to assay the status of the instrument. We haven't done any experiments but I think it could provide valuable QC.

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