Antibody titration on barcoded samples
I am planning a big experiment in which I will barcode and combine 22 PBMC samples, a total of about 70 million cells.
Do any of you have suggestions for how much to increase the staining volume and/or the titrations? I did the titrations on about 3 million cells in 100ul
Previously I combined 20 million cells and increased the titrations 1.33x and the staining volume to a total of 200ul, which worked fine.
For another experiment with 50 million cells the signal intensities already seemed to be significantly lower, so I'm wondering how others deal with this
Thanks!
Marion