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Exosome Detection

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TaReks

Participant

Posts: 1

Joined: Fri Mar 08, 2019 6:33 pm

Post Fri Mar 08, 2019 8:15 pm

Exosome Detection

Hello!

My team and I are looking to investigate exosomes using the CyTOF and looking for some input.

Exosomes are tricky when it comes to CyTOF. It is my understanding that there are issues mainly in the detection. The reason is that often exosomes or other small extracellular vesicles cannot actually “fit” enough antibody or cell-ID reagents on the exosome to be sensed by the detector. The limit of detection is around 350 antibodies per “cell.”

We have discussed a few methods including aggregation or physically expanding the exosomes to allow for more detection signal. However, this specific work is limited.

Any methods or publications out there that might help us?

Any support is greatly appreciated! 8-)

Thanks all!
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dianebender

Participant

Posts: 3

Joined: Mon Feb 11, 2019 1:21 am

Post Sat Mar 09, 2019 6:12 pm

Re: Exosome Detection

Hi Talis,
You could try using a new technology (not CyTOF) called SMCxPro by
MilliporeSigma. It is an immunoassay system (Erenna system) that can do
single molecule counting (SMC) if you have an antibody pair i.e. A capture
antibody and a detection antibody.

Diane
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mleipold

Guru

Posts: 2640

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Sun Mar 10, 2019 1:40 am

Re: Exosome Detection

Hi Talis,

Here's a publication that might help you get started: they looked at synaptosomes by CyTOF.
viewtopic.php?f=10&t=1252
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Fataneh

Participant

Posts: 1

Joined: Thu Nov 05, 2020 8:30 pm

Post Thu Nov 05, 2020 8:58 pm

Re: Exosome Detection

hello
I want to ask the possibility of using CyTOF technology for detecting exosomes.

Would appreciated hearing your thought.
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mleipold

Guru

Posts: 2640

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Fri Nov 06, 2020 4:54 pm

Re: Exosome Detection

In addition to the Synaptosome paper mentioned earlier in the thread, there have been a couple papers looking at exosomes:

https://doi.org/10.1016/j.biomaterials.2020.120152

https://doi.org/10.1039/D0NR02344B

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