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CD27 (clone 0323) sensitivity to fixation

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irenesarkar

Participant

Posts: 2

Joined: Wed Mar 21, 2018 3:57 pm

Post Fri Sep 14, 2018 9:04 am

CD27 (clone 0323) sensitivity to fixation

Hi everyone,

I am working with a panel that includes CD27 (clone 0323) and I was informed that it is sensitive to barcoding reagents. Since I am stimulating my cells, I cannot stain with CD27 prior to stimulation and fixation. Has anyone faced any issues with this clone?
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LKJohnston

Participant

Posts: 1

Joined: Thu May 03, 2018 3:29 pm

Post Fri Sep 14, 2018 3:41 pm

Re: CD27 (clone 0323) sensitivity to fixation

I've tried CD27 O323 on fixed cells (using smarttube buffer) and analyzed them with flow cytometry and the results were not good. There was kind of a positive population, but it was much, much dimmer than when staining fresh cells. I advised one of my users to use the L128 clone for his CyTOF experiment (fixed first with smarttube buffer as well) and the staining looked great! I would advise switching clones.
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irenesarkar

Participant

Posts: 2

Joined: Wed Mar 21, 2018 3:57 pm

Post Fri Sep 21, 2018 12:17 pm

Re: CD27 (clone 0323) sensitivity to fixation

Hi,

Thanks a lot for your reply.

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