MHC Tetramer labeling
Dear CyTOFicionados,
I am looking for any information on how to label MHC-Tetramer.
Are you using DVS MaxPar labeling reagents and protocol ? what are the differences and precautions you need to take compare to IgG labeling? What is the protein yield recovery and number of metal atoms you are able to bind to it ? What is the ratio / concentration of polymer / protein you are using for the conjugation ...?
If you have a protocol that you are willing to share or any advices it would be greatly appreciated.
Hugo
I am looking for any information on how to label MHC-Tetramer.
Are you using DVS MaxPar labeling reagents and protocol ? what are the differences and precautions you need to take compare to IgG labeling? What is the protein yield recovery and number of metal atoms you are able to bind to it ? What is the ratio / concentration of polymer / protein you are using for the conjugation ...?
If you have a protocol that you are willing to share or any advices it would be greatly appreciated.
Hugo