p-STAT5: Cytof vs. Calibur
Dear All,
I've recently started phosphlow experiments on the Cytof. I'm looking for p-STAT5 in human PBMC. The cells are stimulated with IL2 100IU/mL for 10 minutes. This always creates a huge p-STAT5 response of 20 to 60% of the CD4 cells. I've not been getting near the response by mass as I do by traditional flow. I've attached a pdf. The same tube(s) are split in 2 after stim, fix, and perm. Then stained for either mass or fc. Is this normal? Should I have lower expectations for mass positivity in terms of frequency?
Ian
I've recently started phosphlow experiments on the Cytof. I'm looking for p-STAT5 in human PBMC. The cells are stimulated with IL2 100IU/mL for 10 minutes. This always creates a huge p-STAT5 response of 20 to 60% of the CD4 cells. I've not been getting near the response by mass as I do by traditional flow. I've attached a pdf. The same tube(s) are split in 2 after stim, fix, and perm. Then stained for either mass or fc. Is this normal? Should I have lower expectations for mass positivity in terms of frequency?
Ian